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Contains Longley's professional and personal correspondence. Major correspondence include Aaron Klung and J.D. Bernal. Materials date from 1961 to 1987.

Contains Longley's materials pertaining to the Department of Anatomy at Duke University. Major subjects include the Research Award Committee and the Optical Methods Course. Materials date from 1971 to 1986.

Contains grant materials pertaining to Longley's research while at Duke University. Grant applicants include Longley, J. D. Robertson, and M. Reedy. Materials date from 1975 to 1986.